Dynamic binding capacity (DBC) indicates the quantity of a specific biomolecule that can be bound during a chromatography purification step under a specific set of process conditions. Determination and optimization of this value facilitates the development of efficient and productive chromatography operations. While the same general method is used to determine the DBC for many different biomolecules, matrices, and chromatography operations, all of these variables can influence which process parameters are important. Thus, there are many different factors that must be taken into consideration and challenges that must be overcome when optimizing DBCs and ultimately chromatographic performance.
