Search results for " protein L" in Articles / App Notes
Article
Cleaning Protein A Resins with 1 M NaOH
With increasing mAb titers, the impurity level of the cell culture feed increases. If not removed, these impurities can build up on the protein A resin and start to leach into the process material i…
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Affinity chromatography Handbook: Vol. 2 Tagged Proteins
This handbook is intended for those interested in the expression and purification of recombinant proteins. The use of recombinant proteins has increased greatly in recent years, as has the wealth of…
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Handbook: Design of Experiments in Protein Production and Purification
Design of experiments (DoE) is a technique for planning experiments and analyzing the information obtained. The technique allows us to use a minimum number of experiments, in which we systematically v…
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Antibody Fragments and Their Purification by Protein L Affinity Chromatography
Antibodies and related proteins comprise one of the largest and fastest-growing classes of protein pharmaceuticals. A majority of such molecules are monoclonal antibodies; however, many new entities…
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Protein L affinity resin is ready to move to manufacturing
The MabSelect™ VL protein L resin now offers improved alkaline stability compared to Capto™ L resin and a two-fold or more increase in dynamic binding capacity (DBC) in certain applications. After com…
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Capacity and Performance of a Next Generation Protein A Chromatography Resin
MabSelect™ PrismA is a protein A resin of which both the ligand and the base matrix have been optimized. The resin exhibits improved capacity over its predecessor MabSelect SuRe™ products, while maint…
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High Throughput Protein Purification at a Glance
Within the Human Protein Atlas project, scientists are using ÄKTA systems to purify up to 180 protein samples per week. Frida Henningson Johnson, Annelie Cajander, and Anne-Sophie Svensson sha…
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Protein Purification Questions and Answers from ÄKTA Club Web Chat
Do you have recurring discussions in your lab that you need input on? Or do you need support to get on with your protein purification? Around 40 protein purifiers joined our latest ÄKTA club we…
Article
High Throughput Protein Purification at a Glance
Within the Human Protein Atlas project, scientists are using ÄKTA systems to purify up to 180 protein samples per week. The project uses antibodies to study the localization of proteins in huma…
Article
Process development using a nonaffinity method vs Cytiva™ Protein Select™ resin
In this study, we compared two approaches for purifying a recombinant protein that does not have an affinity binding partner.
The first method is using nonaffinity chromatography steps (AI…