Search results for " Cleaning-in-place"
Article
Efficient Cleaning-In-Place Methods for Protein-Based Antibody Affinity Chromatography Resins
This whitepaper discusses cleaning of affinity resins intended for use in the purification of monoclonal antibodies and antibody fragments, for example, Fab fragments. Various cleaning strategies and …
Article
Developing Cleaning-in-Place Protocols
Cleaning conditions for chromatography media can be screened in a couple of working days using 96-well microtitre plate formats. A study with a Protein A resin illustrates the approach in this appli…
Article
Strategizing for Rapid Changeovers in Biologics Manufacturing
Several activities must be performed in a facility to avoid any risk of cross-contamination between Product A and Product B, in particular via a thorough cleaning-in-place of all equipment surfaces in…
Article
Development and Future of Protein A Chromatography Technology: Q&A with Jonathan Royce, Business Leader Chromatography Resins, Cytiva
This week Process Development Forum talks about Protein A with Jonathan Royce, business leader for Cytiva chromatography resins.
mAb purification platforms are very well established, and …
Article
Efficient Sodium Hydroxide Sanitization with a Novel Procedure of AxiChrom™ Columns Packed with Chromatography Resin
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Sodium hydroxide (NaOH) cleaning-in-place is the widely accepted method of sanitization employed by biopharmaceutical manufacturers to avoid the excessive costs and delays of a…
Article
Sanitization Opportunities for Protein A Resins
Here, we describe an approach to safely sanitize our new generation bioprocess protein A affinity resin for antibody purification—MabSelect PrismA—with 30 mM PAA combined with NaOH cleaning-in-place r…
Article
A Look at the Affinity Chromatography Landscape
Protein A is the most sensitive resin to cleaning-in-place and sanitization processes, and one key issue has been the development of more robust versions that can withstand high pH washes to prevent b…
Poster
A Next Generation Protein A Chromatography Resin for Improved Productivity and Bioburden Control
mAb production using a Protein A capture step has followed a highly successful synergistic path the last 30 years. There are, however, remaining challenges.
• The increased upstream titers in mA…
Article
Single-use technologies in downstream process intensification
In bioprocessing, thorough cleaning is critical in order to take full benefit of the resin by removing impurities. When using conventional chromatography solutions, cleaning and cleaning validation …
Article
Comparing Protein A Resins for Monoclonal Antibody Purification
In addition, Protein A resins with the ability to withstand repeated cleaning-in-place (CIP) with low-cost sodium hydroxide (NaOH) considerably improves process economics.
Protein A Resins …