Protein L resins bind to kappa light chain and are often used to purify antibody fragments such as dAbs and Fabs — but they can also be used to remove product-related impurities from bispecific antibodies (bsAb) at capture.
This video presents a study of the effects of different buffer substances, mono-, di-, and tri-carboxylic acids, and concentrations on elution pH, resolution between hetero- and homodimers for bsAb’s, and removal of light chain from mAbs during elution when using MabSelect™ VL protein L resin. The video includes results of these experiments and a discussion of how buffer screening enables improvements in terms of increased elution pH and resolution.